Immunofluorescence databases

Database 1

Studies examining BrdU-NeuN co-labeling in the neocortex of adult mammals have produced conflicting results.  We have found BrdU-NeuN positive cells in the adult neocortex while others have not observed double labeled cells.  One possible reason for these differences is that studies use different staining procedures to visualize BrdU-NeuN in neocortical tissue.   To test this possibility, we stained rat and monkey tissue using two common protocols for BrdU-NeuN labeling.  The protocols produced NeuN staining of differing quality – methods associated with the negative reports yielded reduced NeuN signal to noise in the neocortex.  The protocols and quantitative data on signal to noise ratio for NeuN arising from this study are posted under protocols. Database 1 contains confocal images used in the quantitative analysis of NeuN signal using the two protocols. 

Database 2

This database contains confocal images of BrdU-NeuN positive cells from the prefrontal cortex of three adult male macaques. Orthogonal views of these double-labeled cells are shown.   

Database 3

This database contains confocal images of BrdU-NeuN positive cells from the anterior neocortex of two young adult rats.